PNGase F
Recombinant Glycosidase Cloned from <i>Elizabethkingia miricola</i>
- Use to determine protein glycosylation status and location
- Cleaves N-linked oligosaccharides between innermost GlcNAc and asparagines from N-linked glycoproteins
- Will not remove oligosaccharides found on plant glycoproteins
- Supplied at a concentration of 10u/μl
Catalog Number:
Size
Catalog Number: V4831
PNGase F, Recombinant Glycosidase
PNGase F is a recombinant glycosidase cloned from Elizabethkingia miricola and overexpressed in E. coli. PNGase F has a molecular weight of 36kDa. PNGase F catalyzes the cleavage of N-linked oligosaccharides between the innermost GlcNAc and asparagine residues of high mannose, hybrid and complex oligosaccharides from N-linked glycoproteins (see figure). PNGase F will not remove oligosaccharides containing Alpha-(1,3)-linked core fucose commonly found on plant glycoproteins.
Unit Definition: One unit of PNGase F will catalyze the deglycosylation of 1 nanomole of denatured Ribonuclease B (RNase B) in one minute at 37°C. One Promega unit is equal to 1 IUB milliunit.
Molecular Weight: PNGase F has a molecular weight of approximately 36kDa.
Physical Form: PNGase F is supplied as a liquid in 20mM Tris-HCl (pH 7.5 at 25°C), 50mM NaCl and 5mM EDTA at a concentration of 10,000u/ml.
Applications- Characterizing whether the protein is glycosylated
- Determining the location of glycosylation on the protein
- Characterizing the glycan structure
- Protein trafficking
Twenty micrograms of various denatured glycoprotein standards were treated with 10 units of Promega recombinant or native PNGase F for 1 hour at 37°C. Gel shifts indicating deglycosylation were monitored by SDS-PAGE.
Protocols
Complete Protocol
Specifications
Catalog Number:
What's in the box?
| Item | Part # | Size | Concentration |
|---|---|---|---|
PNGase F |
V483A | 1 × 500 units | 10u/μl |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
Resources
No related resources available
Related Products
Similar Products
Endoglycosidase H
Characterize protein glycosylation in applications such as monitoring protein trafficking and determining glycan location and structure by mass spectrometry.
V4871, V4875
Fetuin
Glycoprotein with O-linked and N-linked glycosylation sites; ideal control substrate to monitor PNGase F or Endo H activity.
V4961
Frequently Used With
IdeS and IdeZ Proteases
IgG-degrading enzymes that cleave at a single site below the hinge region.
V7511, V7515, V8341, V8345
Trypsin Gold, Mass Spectrometry Grade
Maximum digest specificity with extreme resistance to autolytic digestion.
V5280
ProteaseMAX™ Surfactant, Trypsin Enhancer
For improved in-gel digestion and protein solubilization.
V2071, V2072
AccuMAP® Low pH Protein Digestion Kits
Protein digestion at low (mildly acidic) pH to suppress deamidation and disulfide bond scrambling.
VA1040, VA1050
