NanoBRET™ TE 590 Dyes Technical Manual
Instructions for Use of Product(s)
N5000, N5001, N5100, N5200, N5201, N5300, N5301
Literature # TM697
In this technical manual, we describe the use of the NanoBRET™ TE 590 Dyes to develop novel cell-permeable tracers and the considerations for optimizing conditions for intracellular target engagement assay. The NanoBRET™ TE 590 Dyes have consistently demonstrated robust cell permeability and optimal spectral overlap with NanoLuc® for energy transfer. These dyes contain either an amine-reactive succinimidyl ester (SE) group or an azide group that enables the use of Click chemistry to couple the dye to compounds with a reactive alkyne group. In addition, three linker variants of the NanoBRET™ 590 SE dyes are available, allowing a panel of tracer candidates to be prepared from a common intermediate and providing the structural diversity needed to ensure the highest probability of success and, if required, to guide further optimization of tracer development.
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